NYU脊椎冲击损伤仪-脊椎冲击损伤模型制作

     由NYU(W.M. Keck Center for Collaborative Neuroscience, Rutgers the State University of New Jersey)  出品,  在全世界很受歡迎。其產品目前有分兩款，一是model I，可以接電腦，二是model II，可以不接電腦，這兩款大/小鼠均可以使用，且發表論文也非常多，在脊椎損傷研究中,有超過50的論文都是用NYU impactor來發表的。

Overview

The MASCIS Impactor is a device designed to deliver graded reproducible spinal cord contusions in rats.

Developed over ten years ago, the Impactor is part of a well-defined rodent spinal cord injury model that is used in over 100 laboratories around the world. Most of the recommended procedures for the Impactor are based on experience with the model and work done by the Multicenter Animal Spinal Cord Injury Study (MASCIS). The Impactor is now in its third generation with many improvements over previous models.

Introduction

The MASCIS Impactor, formerly called the NYU Impactor, was developed in 1991 by Drs. John Gruner, Carl Mason, and Wise Young. It is now used in laboratories throughout the world in their spinal cord injury studies. The device precisely

measures the movement of a 10-gram rod  dropped 12.5, 25.0, or 50.0 mm onto the T9-10 spinal cord exposed by laminectomy. In addition, the device measures movement of the spinal column at the impact site, displays the trajectory of the falling rod, and measures the impact velocity (ImpV), cord compression distance (Cd), cord compression time (Ct), and cord compression rate (Cr). These impact parameters correlate with each other and spinal cord lesion volumes (estimated from tissue Na and K concentrations) and locomotor recovery (BBB scores).

In  addition, NYU Impactor allows the end user to measure below parameter:

Impact velocity (ImpV)

cord compression distance (Cd)

cord compression time (Ct)

cord compression rate (Cr)

Clamping systems are available for both rat and mice. A clamping system is necessary for NYU Impactor. When Using Clamping system, a CS-tie device is needed.

Size

The MASCIS Impactor is 17” high x 12” deep and 10” wide.

It weighs approximately 11 pounds.

           The MASCIS Impactor is a device designed to deliver graded reproducible spinal cord contusions in rats. Developed over ten years ago, the Impactor is part of a well-defined rodent spinal cord injury model that is used in over 100 laboratories around the world. In addition, more than 50 of recent publications on spinal cord injury research used the MASCIS Impactor. Most of the recommended procedures for the Impactor are based on experience with the model and work done by the Multicenter Animal Spinal Cord Injury Study (MASCIS). 

              The Impactor is now in its third generation with many improvements over previous models. It is available in a model with data recording capability (description and picture links) which requires a Pentium computer. It is also available in a basic model (The Rutgers Impactor) which only does the impact, not the recording of data.

             Clamping systems are available for both rat and mouse.  A clamping system is necessary for the Rutgers basic model and serves as a functional enhancement for the MASCIS model.   When using a clamping system with the MASCIS model, a CS-tie device also is needed.

1.  Serial Changes in Bladder, Locomotion, and in Levels of Neurotrophic Factors in Rats with Spinal Cord Contusion.

Hyun JK, Lee YI, Son YJ, Park JS.

J Neurotrauma. 2009 Feb 9.

 Dankook University College of Medicine, Rehabilitation Medicine, San 16-5 Anseo-dong, Cheonan, Korea, Republic of, 330-714, 82-41-550-6640, 82-41-551-7062; rhhyun@dankook.ac.kr.

The aims of this study were to evaluate the evolution of the neurogenic bladder after spinal cord contusion, and to correlate changes in bladder function with locomotor function and levels of neurotrophic factors. The MASCIS impactor was used to cause a mild contusion injury of the lower thoracic spinal cord of Sprague-Dawley rats. Rats were divided into four groups according to the length of time from injury to sacrifice, at 4, 14, 28, and 56 days after injury. Gait analysis was performed each week, and urodynamic study was performed just before sacrifice. Basso, Beattie, and Bresnahan (BBB) and coupling scores showed gradual recovery, as did the urinary voiding pattern and bladder volume; some parameters of micturition reached normal ranges. Brain-derived neurotrophic factor (BDNF) levels in the spinal cord, as detected by enzyme-linked immunosorbent assay, decreased with time, whereas neurotrophin-3 (NT-3) levels remained unchanged. The micturition pattern, bladder volume and locomotor function continued to recover during the time of observation; BDNF levels in the spinal cord and bladder were inversely correlated with BBB scores and the restoration of bladder volume. We conclude that urodynamic changes in the bladder correlate with locomotion recovery but not with the levels of BDNF or NT3 after modified mild contusion injury in rats.

2:      A re-assessment of minocycline as a neuroprotective agent in a rat spinal cord contusion model.

Pinzon A, Marcillo A, Quintana A, Stamler S, Bunge MB, Bramlett HM, Dietrich WD.

Brain Res. 2008 Dec 3;1243:146-51. Epub 2008 Sep 24.

3:     The role of cation-dependent chloride transporters in neuropathic pain following spinal cord injury.

Cramer SW, Baggott C, Cain J, Tilghman J, Allcock B, Miranpuri G, Rajpal S, Sun D, Resnick D.

Mol Pain. 2008 Sep 17;4:36.

4:     Novel combination strategies to repair the injured mammalian spinal cord.

Bunge MB.

J Spinal Cord Med. 2008;31(3):262-9. Review.

5:    A re-assessment of erythropoietin as a neuroprotective agent following rat spinal cord compression or contusion injury.

Pinzon A, Marcillo A, Pabon D, Bramlett HM, Bunge MB, Dietrich WD.

Exp Neurol. 2008 Sep;213(1):129-36. Epub 2008 Jul 14.

6: B1 and TRPV-1 receptor genes and their relationship to hyperalgesia following spinal cord injury.

DomBourian MG, Turner NA, Gerovac TA, Vemuganti R, Miranpuri GS, Türeyen K, Satriotomo I, Miletic V, Resnick DK.

Spine. 2006 Nov 15;31(24):2778-82.

7:     Endothelial cell loss is not a major cause of neuronal and glial cell death following contusion injury of the   spinal cord.

Casella GT, Bunge MB, Wood PM.

Exp Neurol. 2006 Nov;202(1):8-20. Epub 2006 Jul 26.

8:  Recovery of function following grafting of human bone marrow-derived stromal cells into the injured spinal cord.

Himes BT, Neuhuber B, Coleman C, Kushner R, Swanger SA, Kopen GC, Wagner J, Shumsky JS, Fischer I.

Neurorehabil Neural Repair. 2006 Jun;20(2):278-96.

9:   Mechanical and cold allodynia in a rat spinal cord contusion model.

Yoon YW, Dong H, Arends JJ, Jacquin MF.

Somatosens Mot Res. 2004 Mar;21(1):25-31.

10: Spinal cord contusion models.

Young W.

Prog Brain Res. 2002;137:231-55. Review.