PCR Array

心血管疾病PCR芯片可用于研究与心血管疾病相关的84个基因的表达情况。 The Human Cardiovascular Disease RT2 Profiler PCR Array profiles the expression of 84 genes linked to cardiac disease. Cardiovascular disease is the most important cause of morbidity and mortality in developed countries, causing twice as many deaths as cancer in the United States. Multiple genetic and environmental factors, as well as the interactions between them, increase the risk for developing major cardiovascular diseases such as coronary artery disease (CAD), myocardial infarction (MI), and congestive heart failure (CHF), to name a few. The underlying pathogenic mechanisms for these disorders are still largely unknown, but observed gene expression changes may play a central role in the development and progression of cardiovascular disease. Microarray studies have characterized gene expression patterns in diseased and non-diseased patients leading to the identification of unique subsets of genes associated with the cardiac disease process. The genes profiled with this array play roles in molecular processes such as apoptosis, cardiac remodeling, cell cycle, cell growth, stress and immune responses, transcriptional regulation, and signal transduction. Genes encoding sarcomere structural proteins are represented as well. A set of controls present on each array enables data analysis using the ΔΔCT method of relative quantification and assessment of reverse transcription performance, genomic DNA contamination, and PCR performance. Using real-time PCR, research studies can easily and reliably analyze the expression of a focused panel of genes related to cardiovascular disease with this array. Apoptosis: CCL2, MAOA, NDUFB5, NPPA, NPPB, NPR1, PDE3A, SNCA, THBS2, UBB, ZYX. Cardiac Remodeling: AEBP1, ANXA4, COL11A1, COL1A1, COL3A1, DCN, DMD, F2R, FN1, GJA1, MMP13, MT1X, REN, RTN4, SERPINA3, TNNI3, TNNT2. Cell Cycle: CCND1, CDKN1B, G0S2, RARRES1. Cell Growth: ACE, SFRP4, SPOCK1, CTGF, PTN. Sarcomere Structural Proteins: ACTC1, CRYAB, CRYM, MYH10, MYH6, NEBL, POSTN. Signal Transduction: DUSP6, PDE3B, PDE7A, RASSF1, FRZB, HMGCL, HMGCR, MAPK1, MAPK8, PDE5A, SLC12A1, ADRA1A, ADRA1B, ADRA1D, ADRB1, ADRB2, ADRB3, AGTR1, NPR2, NPR3, AR, EPOR, NR3C1, NR3C2. Stress & Immune Response: C6, CCL2, CCL11, CCL18, S100A1. Transcriptional Regulation: CREB5, CREM, ENAH, HMGN2, KLHL3, MSI2, NFIA, NKX2-5, STAT1, TCF4. Transporters: ATP2A2, ATP5A1.