APOA1 (Human) Native Protein
产品名称: APOA1 (Human) Native Protein
英文名称: APOA1 (Human) Native Protein
产品编号: P5326
产品价格: 0
产品产地: 台湾
品牌商标: Abnova
更新时间: null
使用范围: null
亚诺法生技股份有限公司(Abnova)
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- Specification
- Product Description:
- Human APOA1 native protein from human plasma.
- Host:
- Human
- Form:
- Lyophilized
- Preparation Method:
- High density lipoproteins (HDL) are isolated from fresh, pooled human plasma by ultracentrifugation. The HDL are delipidated and the APOA1 is purified by chromatography in 6M urea.
- Purification:
- Ultracentrifugation and chromatography
- Purity:
- ≥ 98% by SDS-PAGE
- Storage Buffer:
- Lyophilized from 0.01 M ammonium bicarbonate
- Storage Instruction:
- Store at -20°C or lower on dry atmosphere.
After reconstitution with 1 mL of 5 M guanidine-HCl, store at 2-8°C no longer than 4 weeks.
- Note:
- Universal Precautions. Safe laboratory practices should be used when handling this material.
- Background Information:
- Apolipoprotein A1, which has a molecular weight of 28 kDa and a pI of 5.6, activates LCAT by catalyzing the esterification of cholesterol. Apolipoproteins are soluble in aqueous solutions. However, due to their amphipathic structure, apolipoproteins self-associate into oligomers at high protein concentrations. At concentrations above 1 mg/mL, there may be little or no free monomer in the solution. Apolipoproteins exist as monomers in buffers containing 5 M guanidine-HCl or 6M urea.
- Application Image
- SDS-PAGE
- Entrez GeneID:
- 335
- Gene Name:
- APOA1
- Gene Alias:
- MGC117399
- Gene Description:
- apolipoprotein A-I
- Gene Ontology:
- Hyperlink
- Gene Summary:
- This gene encodes apolipoprotein A-I, which is the major protein component of high density lipoprotein (HDL) in plasma. The protein promotes cholesterol efflux from tissues to the liver for excretion, and it is a cofactor for lecithin cholesterolacyltransferase (LCAT) which is responsible for the formation of most plasma cholesteryl esters. This gene is closely linked with two other apolipoprotein genes on chromosome 11. Defects in this gene are associated with HDL deficiencies, including Tangier disease, and with systemic non-neuropathic amyloidosis. [provided by RefSeq
- Other Designations:
- OTTHUMP00000069346,OTTHUMP00000069347,OTTHUMP00000069348,apolipoprotein A1
- Interactome
- Gene Pathway
- Related Disease
- Albuminuria
- Alzheimer Disease
- Alzheimer disease
- Anemia, Sickle Cell
- Anemia, sickle cell
- Angina Pectoris
- Aortic Valve Stenosis
- Atherosclerosis
- Atherosclerosis
- Brain Ischemia
- Calcinosis
- Cardiovascular Diseases
- Carotid Artery Diseases
- Cerebral Hemorrhage
- Cerebral Infarction
- Cerebrovascular Accident
- Cerebrovascular Disorders
- Chorioamnionitis
- Cleft Lip